Patients won't be encouraged to stay in bed at Dubbo Hospital from September 1.
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Instead, they will be urged to get dressed in their own "comfy day clothes" and get moving.
Pyjamas will become nighttime attire only during a 90-day challenge at the hospital.
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The hospital is taking part in #EndPJParalysis, a movement founded by the United Kingdom's Professor Brian Dolan OBE and supported by health professionals across the globe.
It aims to reduce the immobility, muscle deconditioning and dependency experienced by bed-bound hospital patients, while protecting their cognitive function, social interaction and dignity.
Western NSW Local Health District project lead, Rachael Hallam, says the international campaign focuses on the mental and physical benefits of patients wearing day clothes and keeping active during their stay in hospital.
"Wearing normal day clothes instead of pyjamas helps makes patients feel more confident and independent," she said.
"It also makes it easier to keep active which reduces the risk of muscle weakness, falls and pressure sores.
"This is especially important for the elderly.
"For people over 80, one week in a hospital bed can lead to a loss of 1.5 kilograms of muscle and 10 per cent loss of general fitness."
During the challenge, Dubbo Hospital staff aim to "get as many patients up, dressed and moving each morning as possible".
"Patients can support the campaign and improve their health by bringing comfy day clothes to hospital or asking family, friends or carers to bring suitable clothes" Ms Hallam said.
Six other hospitals in the health district are taking part in the of the #EndPJParalysis challenge.
They are at Bathurst, Orange, Mudgee, Parkes, Forbes and Cowra.