DUBBO now has two world records under its belt after more than 1000 women and their bikes gathered on Saturday.
In 2014 the 2WheelBabes set a record for most women on bikes (221) at Ballina for the annual Babe Raid, but this record was beaten in August 2015 by a group of 246 women in the UK.
Organiser Debb Dagger was hoping to reclaim the record, while also surpassing the most women at a motorcycle meet, which stood at 681.
The numbers were far higher than expected with 1002 women riders turning up to Dubbo, and thousands gathering in total.
Ms Dagger said she was very, very humbled by the turnout.
There were people lining every street they road through on Saturday, she said.
As well as breaking a world record, Ms Dagger said the event was also about raising a greater awareness of female riders.
"Female riders are underestimated sometimes by the wider community. We've all got to get together and support each other," she said.
"I know there are women who have met people from different states, so if any of those women want to travel long distances they'll have somewhere to stay, someone to ride with."
The record may have only just been broken but Ms Dagger has already promised that if the title is taken away she would "endeavour to re-beat it".
Ms Dagger said she wanted to thank everybody who attended the event, the sponsors and the Dubbo community for its support.
More information on the 2WheelBabes can be found at www.2wheelbabes.com.