Personal trainer Emma Kearines said the enthusiastic group would leave Dubbo at 2am on Sunday then walk the 14-kilometre course shortly after their arrival in Sydney.

"My mum Vicki is helping out and my Dad Greg will drive us all there in a bus - we should get to Sydney about half an hour before the event starts and then we'll leave just after it finishes to come home the same night," she said.

Many Dubbo residents would have spotted the ladies completing eight walks of similar distance in the weeks leading up to the event.

Ms Kearines said the group was feeling particularly inspired by the Olympics at the moment, and would make sure they got plenty of rest on the bus trip on the way down so they were in tip-top shape ahead of the walk.

The idea for the trip had come about after the ladies decided they wanted a fitness-related get-together.

"I wanted to give the ladies (who I train) something to work towards, and at the same time help raise money for a charity, which I do every year," she said.

Ms Kearines said the charity of choice this year was the Dubbo Prostate Cancer Support Group.

"We've raised money for breast cancer in the past, and as we all have husbands, partners, brothers, fathers and friends who are men, we thought it was time to do something for them," she said.

"And specifically we wanted the money to stay in the local area."

The group had also been buoyed by generous support from local company Robert Holmes Transport, who had helped the girls buy singlets they would wear on the run that would promote the Dubbo Prostate Cancer Support Group.

Ms Kearines said anyone who would like to sponsor the walkers can contact her on 0407 834 568.